Ca2+-regulated structural changes in troponin
نویسندگان
چکیده
منابع مشابه
Ca(2+)-regulated structural changes in troponin.
Troponin senses Ca2+ to regulate contraction in striated muscle. Structures of skeletal muscle troponin composed of TnC (the sensor), TnI (the regulator), and TnT (the link to the muscle thin filament) have been determined. The structure of troponin in the Ca(2+)-activated state features a nearly twofold symmetrical assembly of TnI and TnT subunits penetrated asymmetrically by the dumbbell-shap...
متن کاملStructural changes induced in Ca2+-regulated myosin filaments by Ca2+ and ATP
We have used electron microscopy and proteolytic susceptibility to study the structural basis of myosin-linked regulation in synthetic filaments of scallop striated muscle myosin. Using papain as a probe of the structure of the head-rod junction, we find that this region of myosin is approximately five times more susceptible to proteolytic attack under activating (ATP/high Ca2+) or rigor (no AT...
متن کاملChanges in myofibrillar activation and troponin C Ca2+ binding associated with troponin T isoform switching in developing rabbit heart.
Postnatal development of the mammalian heart is associated with changes in the population of isoforms of the thin filament proteins. We correlated the change in thin filament proteins, which occur in rabbit hearts between 5 days and 22 days of age, with changes in Ca2+ dependence of myofibrillar ATPase activity, force generation, and troponin C Ca2+ binding. The preparations derived from the 5-...
متن کاملFluorescent Protein-Based Ca2+ Sensor Reveals Global, Divalent Cation-Dependent Conformational Changes in Cardiac Troponin C
Cardiac troponin C (cTnC) is a key effector in cardiac muscle excitation-contraction coupling as the Ca2+ sensing subunit responsible for controlling contraction. In this study, we generated several FRET sensors for divalent cations based on cTnC flanked by a donor fluorescent protein (CFP) and an acceptor fluorescent protein (YFP). The sensors report Ca2+ and Mg2+ binding, and relay global str...
متن کاملSmall molecule regulated dynamic structural changes of human G-quadruplexes
Department of Organic Chemistry, Indian A Jadavpur, Kolkata-700032, India. E-mail: oc Department of Physical Chemistry, Indian A Jadavpur, Kolkata-700032, India Institute of Organic Chemistry and Chemica Center for Biomolecular Magnetic Resonance Frankfurt am Main, Germany † Electronic supplementary information synthetic procedures, characterization d NMR spectra, sm-FRET, shot noise, FCS NMR s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2005
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0408882102